The draft chromosome-level genome assembly of tetraploid ground cherry (Prunus fruticosa Pall.) from long reads
Thomas Wöhner, Ofere Francis Emeriewen, Alexander Wittenberg, Harrie Schneiders, Ilse Vrijenhoek, J. Halász, K. Hrotkó, Katharina J. Hoff, Lars Gabriel, Janne Lempe, Jens Keilwagen, Thomas Berner, Mirko Schuster, Andreas Peil, J. Wünsche, Stephan Kropop, Henryk Flachowsky
Abstract
Cherries are stone fruits and belong to the economically important plant family of Rosaceae with worldwide cultivation of different species. The ground cherry, Prunus fruticosa Pall., is an ancestor of cultivated sour cherry, an important tetraploid cherry species. Here, we present a long read chromosome-level draft genome assembly and related plastid sequences using the Oxford Nanopore Technology PromethION platform and R10.3 pore type. We generated a final consensus genome sequence of 366 Mb comprising eight chromosomes. The N50 scaffold was ~44 Mb with the longest chromosome being 66.5 Mb. The chloroplast and mitochondrial genomes were 158,217 bp and 383,281 bp long, which is in accordance with previously published plastid sequences. This is the first report of the genome of ground cherry (P. fruticosa) sequenced by long read technology only. The datasets obtained from this study provide a foundation for future breeding, molecular and evolutionary analysis in Prunus studies.