Metagenomic Sequencing To Detect Respiratory Viruses in Persons under Investigation for COVID-19
Ahmed Babiker, Heath L. Bradley, Victoria Stittleburg, Jessica Ingersoll, Autum Key, Colleen S. Kraft, Jesse J. Waggoner, Anne Piantadosi
Abstract
Broad testing for respiratory viruses among persons under investigation (PUIs) for SARS-CoV-2 has been performed inconsistently, limiting our understanding of alternative viral infections and coinfections in these patients. RNA metagenomic next-generation sequencing (mNGS) offers an agnostic tool for the detection of both SARS-CoV-2 and other RNA respiratory viruses in PUIs. Here, we used RNA mNGS to assess the frequencies of alternative viral infections in SARS-CoV-2 RT-PCR-negative PUIs ( n = 30) and viral coinfections in SARS-CoV-2 RT-PCR-positive PUIs ( n = 45).
Topics & Concepts
MetagenomicsVirologyBiologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)LimitingRNACoinfection2019-20 coronavirus outbreakRespiratory systemSars virusVirusMedicineGeneGeneticsOutbreakPathologyInfectious disease (medical specialty)EngineeringDiseaseAnatomyMechanical engineeringRespiratory viral infections researchSARS-CoV-2 detection and testingViral gastroenteritis research and epidemiology