Different Methods Used For Determination of Vitamin C: A Review
Swati Raman, Anjali Sehrawat, Rupa Upadhyay, Swati Singh, Aditi Verma, Shreya Jain Aarushi, Kanika Pooja Raizada, Anupa Siddhu, Aparna Aggarwal
Abstract
Vitamin C or ascorbic acid is essential for human life and is required for a range of physiological functions in the human body. It is naturally present in fresh fruits and vegetables. Ascorbic Acid (AA) is a natural and powerful water-soluble antioxidant associated with long-lasting food products. It has been widely used in the pharmaceutical, chemical, cosmetic and food industry as an antioxidant. Therefore, there is a need to find an accurate and reliable method for measuring the amount of ascorbic acid in a sample. The purpose of this review is to discuss different methods used for determination of vitamin C. The different methods used for the determination of vitamin C are UV-spectrophotometry, high-performance liquid chromatography (HPLC), dye titration method, iodometric titration, iodimetric titration, enzymatic methods, fluorometry and voltammetry.