CYTOTOXIC EFFECT OF CHLOROFORM EXTRACTS FROM TANACETUM VULGARE, T. MACROPHYLLUM AND T. CORYMBOSUM ON HELA, A375 AND V79 CELL LINES
Bianca Ivănescu
Abstract
The effect of Tanacetum extracts on cell viability was assessed by MTT method on HeLa (human cervical epithelioid carcinoma), A375 (human malignant melanoma) and V79 (Chinese hamster pulmonary fibroblasts). Apoptosis, cell cycle analysis, and genotoxicity tests were performed to identify the possible mechanism of action. Also, the antimicrobial activity was investigated, and LC-MS analysis of extracts was carried out. Tanacetum extracts substantially reduced the viability of all tested cells, normal cells being more sensitive than cancer cells. T. vulgare and T. macrophyllum extracts induced apoptosis in normal cells V79, while none of the extracts induced apoptosis on HeLa or A375 neoplastic cells. T. vulgare extract arrested cell cycle progression of V79 and A375 cells at the G2/M phase and exhibited mild genotoxicity on melanoma cells A375. All extracts showed moderate antimicrobial activity against tested Gram-positive bacteria and fungi.