Cell Wall Composition of Hemp Shiv Determined by Physical and Chemical Approaches
Maya-Sétan Diakité, Hélène Lenormand, Vincent Lequart, Santiago Arufe, Patrick Martin, Nathalie Leblanc
Abstract
The use of agricultural by-products in the building engineering realm has led to an increase in insulation characteristics of biobased materials and a decrease in environmental impact. The understanding of cell wall structure is possible by the study of interactions of chemical compounds, themselves determined by common techniques like Van Soest (VS). In this study, a global method is investigated to characterise the cell wall of hemp shiv. The cell wall molecules were, at first, isolated by fractionation of biomass and then analysed by physical and chemical analysis (Thermal Gravimetric Analysis, Elementary Analysis, Dynamic Sorption Vapor and Infra-Red). This global method is an experimental way to characterise plant cell wall molecules of fractions by Thermal Gravimetric Analysis following by a mathematical method to have a detailed estimation of the cell wall composition and the interactions between plant macromolecules. The analyzed hemp shiv presents proportions of 2.5 ± 0.6% of water, 4.4 ± 0.2% of pectins, 42.6 ± 1.0% (Hemicellulose-Cellulose), 18.4 ± 1.6% (Cellulose-Hemicellulose), 29.0 ± 0.8% (Lignin-Cellulose) and 2.0 ± 0.4% of linked lignin.