Epigallocatechin-3-Gallate PEGylated Poly(Lactic-Co-Glycolic) Acid Nanoparticles Mitigate Striatal Pathology and Motor Deficits in 3-Nitropropionic Acid Intoxicated Mice
Amanda Cano, Miren Ettcheto, Marta Espina, Carmen Auladell, Jaume Folch, Britta Anna Kühne, Marta Barenys, Elena Sánchez‐López, Eliana B. Souto, María L. García, Patric Turowski, Antoni Camins
Abstract
Aim: To compare free and nanoparticle (NP)-encapsulated epigallocatechin-3-gallate (EGCG) for the treatment of Huntington’s disease (HD)-like symptoms in mice. Materials & methods: EGCG was incorporated into PEGylated poly(lactic-co-glycolic) acid NPs with ascorbic acid (AA). HD-like striatal lesions and motor deficit were induced in mice by 3-nitropropionic acid-intoxication. EGCG and EGCG/AA NPs were co-administered and behavioral motor assessments and striatal histology performed after 5 days. Results: EGCG/AA NPs were significantly more effective than free EGCG in reducing motor disturbances and depression-like behavior associated with 3-nitropropionic acid toxicity. EGCG/AA NPs treatment also mitigated neuroinflammation and prevented neuronal loss. Conclusion: NP encapsulation enhances therapeutic robustness of EGCG in this model of HD symptomatology. Together with our previous findings, this highlights the potential of EGCG/AA NPs in the symptomatic treatment of neurodegenerative diseases.