Systems-Level Analysis of the Global Regulatory Mechanism of CodY in Lactococcus lactis Metabolism and Nisin Immunity Modulation
Hao Wu, Kairen Tian, Jia Feng, Hao Qi, Jianjun Qiao
Abstract
Lactococcus lactis, a species of lactic acid bacteria (LAB) widely used in food fermentation, has been the model strain in genetic engineering, and its application has extended from food to microbial cell factories. CodY is a global regulator in low-G+C Gram-positive bacteria. Its function and direct target genes at the genome-level are little known in L. lactis. In this study, we describe the comprehensive regulation mechanism of CodY. It widely modulated the metabolism of nitrogen and carbohydrate, cell wall synthesis, and nisin immunity in L. lactis F44, and its expression level was regulated by feedback control.
Topics & Concepts
Lactococcus lactisNisinProteomeBiologyRepressorGeneChromatin immunoprecipitationBacteriocinTranscription (linguistics)Transcriptional regulationTranscription factorBiochemistryRegulation of gene expressionActivator (genetics)BacteriaProteomicsRegulatorATP-binding cassette transporterCell biologyGeneticsPeptide sequenceLactococcusElectrophoretic mobility shift assayAmino acidGene expressionRegulator geneLantibioticsProbiotics and Fermented FoodsBacterial Genetics and BiotechnologyMicrobial Metabolic Engineering and Bioproduction