A Nonconventional Archaeal Fluorinase Identified by In Silico Mining for Enhanced Fluorine Biocatalysis
Isabel Pardo, David Bednář, Patricia Calero, Daniel C. Volke, Jiřı́ Damborský, Pablo I. Nikel
Abstract
2 fluorination rate reported to date. Furthermore, we demonstrate enhanced production of fluoronucleotides in vivo in a bacterial host engineered with this archaeal fluorinase, paving the way toward synthetic metabolism for efficient biohalogenation.
Topics & Concepts
In silicoBiocatalysisChemistryBiologyBiochemical engineeringEnzymeComputational biologyCatalysisNanotechnologyBiochemistryMaterials scienceReaction mechanismGeneEngineeringMicrobial Community Ecology and PhysiologyCancer Research and TreatmentsEnzyme Structure and Function