Impact of the methylation classifier and ancillary methods on CNS tumor diagnostics
Zhichao Wu, Zied Abdullaev, Drew Pratt, Hye‐Jung Chung, Shannon Skarshaug, Valerie Zgonc, Candice Perry, Svetlana Pack, Lola Saidkhodjaeva, Sushma Nagaraj, Manoj Tyagi, Vineela Gangalapudi, Kristin Valdez, Rust Turakulov, Liqiang Xi, Mark Raffeld, Antonios Papanicolau‐Sengos, Kayla O’Donnell, Michael Newford, Mark R. Gilbert, Felix Sahm, Abigail K. Suwala, Andreas von Deimling, Yasin Mamatjan, Shirin Karimi, Farshad Nassiri, Gelareh Zadeh, Eytan Ruppin, Martha Quezado, Kenneth Aldape
Abstract
BACKGROUND: Accurate CNS tumor diagnosis can be challenging, and methylation profiling can serve as an adjunct to classify diagnostically difficult cases. METHODS: An integrated diagnostic approach was employed for a consecutive series of 1258 surgical neuropathology samples obtained primarily in a consultation practice over 2-year period. DNA methylation profiling and classification using the DKFZ/Heidelberg CNS tumor classifier was performed, as well as unsupervised analyses of methylation data. Ancillary testing, where relevant, was performed. RESULTS: Among the received cases in consultation, a high-confidence methylation classifier score (>0.84) was reached in 66.4% of cases. The classifier impacted the diagnosis in 46.7% of these high-confidence classifier score cases, including a substantially new diagnosis in 26.9% cases. Among the 289 cases received with only a descriptive diagnosis, methylation was able to resolve approximately half (144, 49.8%) with high-confidence scores. Additional methods were able to resolve diagnostic uncertainty in 41.6% of the low-score cases. Tumor purity was significantly associated with classifier score (P = 1.15e-11). Deconvolution demonstrated that suspected glioblastomas (GBMs) matching as control/inflammatory brain tissue could be resolved into GBM methylation profiles, which provided a proof-of-concept approach to resolve tumor classification in the setting of low tumor purity. CONCLUSIONS: This work assesses the impact of a methylation classifier and additional methods in a consultative practice by defining the proportions with concordant vs change in diagnosis in a set of diagnostically challenging CNS tumors. We address approaches to low-confidence scores and confounding issues of low tumor purity.