Electrochemiluminescence platform for transcription factor diagnosis by using CRISPR–Cas12a <i>trans</i>-cleavage activity
Zhenqiang Fan, Yuedi Ding, Bo Yao, Jiaying Wang, Kai Zhang
Abstract
Herein, we exploited the double-stranded DNA (dsDNA) binding property of transcription factor (TF), combined with the trans cleavage characteristic of CRISPR-Cas12a, for the detection of NF-κB p50.
Topics & Concepts
CRISPRCleavage (geology)ElectrochemiluminescenceTranscription factorComputational biologyDNAChemistryMolecular biologyCombinatorial chemistryBiologyBiochemistryGeneChromatographyDetection limitFracture (geology)PaleontologyAdvanced biosensing and bioanalysis techniquesCRISPR and Genetic EngineeringRNA Interference and Gene Delivery