Correlative light-electron microscopy: integrating dynamics to structure
Andrea G. Marshall, Evan Krystofiak, Steven M. Damo, Antentor Hinton
Abstract
A challenge of classical electron microscopy (EM) modalities is the static and limited view they present of dynamic biological processes. Correlative light and EM (CLEM) pairs the precise imaging of the cellular location in light microscopy (LM) and EM, taking advantage of both methods. Recent advances in protein labeling, fluorescent markers, super-resolution microscopy, and EM technologies have allowed widespread application in modern biological research. Because of the wide versatility of CLEM, it is difficult to describe a single workflow.
Topics & Concepts
CorrelativeElectron microscopeMicroscopyBiophysicsBiological systemComputer scienceComputational biologyChemistryNanotechnologyBiologyPhysicsOpticsMaterials scienceLinguisticsPhilosophyAdvanced Fluorescence Microscopy TechniquesAdvanced Electron Microscopy Techniques and ApplicationsPhotosynthetic Processes and Mechanisms