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Absolute quantification of prokaryotes in the microbiome by 16S rRNA qPCR or ddPCR

Boryana Doyle, Gabriella Z. M. Reynolds, Mai Dvorak, Dylan G. Maghini, Aravind Natarajan, Ami S. Bhatt

2025Nature Protocols19 citationsDOI

Topics & Concepts

MicrobiomeComputational biologyBiologyRibosomal RNAMicrobial genetics16S ribosomal RNAMetagenomicsGeneticsBacteriaGeneGenomics and Phylogenetic StudiesMolecular Biology Techniques and ApplicationsMicrobial Community Ecology and Physiology
Absolute quantification of prokaryotes in the microbiome by 16S rRNA qPCR or ddPCR | Litcius