Identification of the interacting proteins of Bambusa pervariabilis × Dendrocalamopsis grandis in response to the transcription factor ApCtf1β in Arthrinium phaeospermum
Peng Yan, Jiawen Yu, Xinmei Fang, Shuying Li, Shuying Li, Shan Han, Tiantian Lin, Yinggao Liu, Chunlin Yang, Fang He, Tianhui Zhu, Shujiang Li, Shujiang Li
Abstract
Arthrinium phaeospermum is the main pathogen that causes Bambusa pervariabilis × Dendrocalamopsis grandis blight. It secretes the cutinase transcription factor ApCtf1β , which has been shown to play an important role in B. pervariabilis × D. grandis virulence. However, knowledge about the interaction target genes of ApCtf1β in B. pervariabilis × D. grandis remains limited. A cDNA library for the yeast two-hybrid system was constructed from B. pervariabilis × D. grandis shoots after 168 h treatment with A. phaeospermum . The library was identified as 1.20 × 10 7 cfu, with an average insert >1,000 bp in size and a 100% positive rate, providing a database for the subsequent molecular study of the interaction between A. phaeospermum and B. pervariabilis × D. grandis . The yeast two-hybrid (Y2H), bimolecular fluorescence complementation (BiFC), and glutathione-S-transferase (GST) pull-down assays were used to screen for and identify two ApCtf1β interacting target proteins, BDUbc and BDSKL1 , providing a reliable theoretical basis to study the molecular mechanism underlying B. pervariabilis × D. grandis resistance in response to A. phaeospermum , which would, in turn, establish a platform to develop new strategies for the sustainable and effective control of the blight diseases of forest trees.