Recent advances in biological nanopores for nanopore sequencing, sensing and comparison of functional variations in MspA mutants
Huma Aslam Bhatti, Rohil Jawed, İrshad Ali, Khurshid Iqbal, Han Yan, Zuhong Lu, Quanjun Liu
Abstract
porin A (MspA), aerolysin, bacteriophage phi 29 and Outer membrane porin G (OmpG). Herein, we discuss some well-known biological nanopores but emphasize on MspA and compare the effects of site-directed mutagenesis on the detection ability of its mutants in view of the surface charge distribution, voltage threshold and pore-analyte interaction. We also discuss illustrious and latest advances in biological nanopores for past 2-3 years due to limited space. Last but not the least, we elucidate our perspective for selecting a biological nanopore and propose some future directions to design a customized nanopore that would be suitable for DNA sequencing and sensing of other nontrivial molecules in question.