<i>Lactobacillus ingluviei</i> C37 from chicken inhibits inflammation in LPS‐stimulated mouse macrophages
Masami Tsukagoshi, Merisa Sirisopapong, Fu Namai, Minori Ishida, Supattra Okrathok, Suguru Shigemori, Tasuku Ogita, Takashi Sato, Sutisa Khempaka, Takeshi Shimosato
Abstract
Probiotics are growing alternatives to antibiotics, and can contribute to the prevention and treatment of diseases and enhance livestock production. Lactobacillus (L.) ingluviei is a novel probiotic species with growth-enhancement effects; however, this species remains poorly understood, and there have been (to our knowledge) no studies focusing on its immunological effects. Here, we isolated L. ingluviei C37 (LIC37) from chicken and evaluated the bacterium's immunomodulatory properties to explore its probiotic potential. Real-time quantitative PCR and ELISA showed that in vitro exposure of inflammation-stimulated mouse peritoneal macrophages to heat-killed LIC37 led to decreases in tumor necrosis factor-α and interleukin (IL)-6 levels and an increase in IL-10. These findings suggested that LIC37 exerts anti-inflammatory effects by modulating cytokine profiles. This species may be an attractive probiotic bacterial strain for use in animal production.