Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides
Demetra S.M. Chatzileontiadou, Christopher Szeto, Dhilshan Jayasinghe, Stéphanie Gras
Abstract
Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).
Topics & Concepts
Human leukocyte antigenCrystallizationComputational biologyAntigenProtocol (science)PopulationBiologyAntigen presentationCellChemistryImmunologyCell biologyT cellImmune systemBiochemistryMedicinePathologyOrganic chemistryEnvironmental healthAlternative medicinevaccines and immunoinformatics approachesImmunotherapy and Immune ResponsesSARS-CoV-2 and COVID-19 Research