Native mass spectrometric studies of IscSU reveal a concerted, sulfur-initiated mechanism of iron–sulfur cluster assembly
Sophie P. Bennett, Jason C. Crack, Rita Puglisi, Annalisa Pastore, Nick E. Le Brun
Abstract
Time-resolved native mass spectrometry was used to investigate iron–sulfur cluster assembly on IscU. Data revealed a concerted assembly process in which sulfur (S 0 ) transfer must occur first if IscU is in its Zn 2+ -bound form.
Topics & Concepts
BiogenesisSulfurChemistryScaffold proteinCluster (spacecraft)Iron–sulfur clusterCofactorBiochemistryCysteineMechanism (biology)Sulfur metabolismFunction (biology)BiologyGeneticsEnzymeGeneOrganic chemistryProgramming languageSignal transductionEpistemologyComputer sciencePhilosophyMetalloenzymes and iron-sulfur proteinsTrace Elements in HealthMicrobial Fuel Cells and Bioremediation