Targeted imaging of integrins in cancer tissues using photocleavable Ru(<scp>ii</scp>) polypyridine complexes as mass-tags
Jiaying Han, Jing Sun, Shanshan Song, Leonie Beljaars, Geny M. M. Groothuis, Hjalmar P. Permentier, Rainer Bischoff, György B. Halmos, Cornelia J. Verhoeven, Erika R. Amstalden van Hove, Péter Horvatovich, Angela Casini
Abstract
Targeted epitope-based mass spectrometry imaging (MSI) utilizes laser cleavable mass-tags bound to targeting moieties for detecting proteins in tissue sections. Our work constitutes the first proof-of-concept of a novel laser desorption ionization (LDI)-MSI strategy using photocleavable Ru(ii) polypyridine complexes as mass-tags for imaging of integrins αvβ3 in human cancer tissues.
Topics & Concepts
RutheniumChemistryMass spectrometryIntegrinMass spectrometry imagingCombinatorial chemistryCancerBiophysicsBiochemistryChromatographyReceptorBiologyCatalysisGeneticsMass Spectrometry Techniques and ApplicationsAdvanced biosensing and bioanalysis techniquesAdvanced Proteomics Techniques and Applications