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Structure-specific DNA recombination sites: Design, validation, and machine learning–based refinement

Aleksandra Nivina, Maj Svea Grieb, Céline Loot, David Bikard, Jean Cury, Laila Shehata, Juliana Bernardes, Didier Mazel

2020Science Advances26 citationsDOIOpen Access PDF

Abstract

design principles. They can be embedded into virtually any sequence and constitute a unique example of a structure-specific DNA recombination system.

Topics & Concepts

RecombinationIntegronIntegraseDNASequence (biology)Site-specific recombinationComputational biologyDNA sequencingMutagenesisGeneticsBiologyComputer scienceRecombinaseMutationGenePlasmidRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyCRISPR and Genetic Engineering