Combined engineering of l-sorbose dehydrogenase and fermentation optimization to increase 2-keto-l-gulonic acid production in Escherichia coli
Dong Li, Xinglong Wang, Zhijie Qin, Shiqin Yu, Jian Chen, Jingwen Zhou
Topics & Concepts
FermentationChemistrySubstrate (aquarium)Escherichia coliBiochemistryMutantSaturated mutagenesisDirected evolutionMetabolic engineeringStereochemistryYield (engineering)EnzymeBiologyMaterials scienceMetallurgyEcologyGeneMicrobial metabolism and enzyme functionEnzyme Structure and FunctionBiochemical and Molecular Research