Novel multiresistance-mediating integrative and conjugative elements carrying unusual antimicrobial resistance genes in <i>Mannheimia haemolytica</i> and <i>Pasteurella multocida</i>
Anne Kathrin Schink, Dennis Hanke, Torsten Semmler, Julian Brombach, Astrid Bethe, Antina Lübke‐Becker, Kinga Teske, Kerstin Müller, Štefan Schwarz
Abstract
Mannheimia haemolytica and Pasteurella multocida play important roles in the bovine respiratory disease (BRD) complex.1,2 Isolates of these species, harbouring multiresistance-mediating integrative and conjugative elements (ICEs), such as ICEPmu1 and ICEMh1 among others, have been identified in North America,3–6 but not yet in European countries. In 2019, M. haemolytica IMT47952 and P. multocida IMT47951, showing an unusual multiresistance phenotype, were obtained from a fatal case of BRD in Germany. The affected animal was a 3-month-old male Holstein-Friesian fattening calf with an unknown history of antimicrobial pretreatment. Closed genomes of both isolates were generated by hybrid assembly of Illumina MiSeq™ and MinION reads and analysed with particular reference to antimicrobial resistance genes and their potential association with ICEs. The plasmid-free M. haemolytica IMT47952 had a genome size of 2 647 889 bp. It was resistant to tilmicosin, tulathromycin, florfenicol, tetracycline, penicillin and ampicillin, intermediate to...