Morphometric Cell Classification for Single‐Cell MALDI‐Mass Spectrometry Imaging
Klára Ščupáková, Frédéric Dewez, Axel Walch, Ron M. A. Heeren, Benjamin Balluff
Abstract
The large-scale and label-free molecular characterization of single cells in their natural tissue habitat remains a major challenge in molecular biology. We present a method that integrates morphometric image analysis to delineate and classify individual cells with their single-cell-specific molecular profiles. This approach provides a new means to study spatial biological processes such as cancer field effects and the relationship between morphometric and molecular features.
Topics & Concepts
Mass spectrometry imagingMALDI imagingMass spectrometryCellChemistryChromatographyMatrix-assisted laser desorption/ionizationBiochemistryAdsorptionOrganic chemistryDesorptionMass Spectrometry Techniques and ApplicationsMetabolomics and Mass Spectrometry StudiesAdvanced Proteomics Techniques and Applications