Employing non-canonical amino acids towards the immobilization of a hyperthermophilic enzyme to increase protein stability
Hannah J. Switzer, Christina A. Howard, John F. Halonski, Emily M. Peairs, Nolan Smith, Maddy P. Zamecnik, Sanjana Verma, Douglas D. Young
Abstract
non-canonical amino acids. The immobilized enzyme exhibited heightened performance in organic solvents, recyclability, and stability at room temperature for over two years. The incorporation of a non-canonical amino acid afforded a high degree of control over the bioorthogonal immobilization reaction. These results indicate that the specificity conferred by genetic code expansion produces advantages in protein immobilization and broadens the utility of such proteins to non-biological settings.
Topics & Concepts
Sulfolobus solfataricusChemistryAmino acidCarboxylesteraseEnzymeSepharoseEpoxyChromatographyBiochemistryOrganic chemistryArchaeaGeneEnzyme Catalysis and ImmobilizationMolecular Junctions and NanostructuresClick Chemistry and Applications