Intrapulmonary Treatment with Mycobacteriophage LysB Rapidly Reduces Mycobacterium abscessus Burden
Kelley Hurst-Hess, Amanda Walz, Yong Yang, Helen McGuirk, Mercedes Gonzalez‐Juarrero, Graham F. Hatfull, Pallavi Ghosh, Anil K. Ojha
Abstract
Intrinsic and acquired antibiotic resistance in Mycobacterium abscessus presents challenges in infection control, and new therapeutic strategies are needed. Bacteriophage therapy shows promise, but variabilities in M. abscessus phage susceptibility limits its broader utility. We show here that a mycobacteriophage-encoded lysin B (LysB) efficiently and rapidly kills both smooth- and rough-colony morphotype M. abscessus strains and reduces the pulmonary bacterial load in mice. LysB aerosolization presents a plausible treatment for pulmonary M. abscessus infections.
Topics & Concepts
Mycobacterium abscessusMedicineMycobacteriumMycobacterium InfectionsMicrobiologyVirologyBiologyTuberculosisPathologyBacteriophages and microbial interactionsMycobacterium research and diagnosisMicrobial infections and disease research