circNFIX facilitates hepatocellular carcinoma progression by targeting miR-3064-5p/HMGA2 to enhance glutaminolysis.
Erhui Xiao, Dongmei Zhang, Weili Zhan, Hui Yin, Li Ma, Junfeng Wei, Yi Kang, Zhongshan Mao
Abstract
was detected using xenograft assay. Our results showed that circNFIX was remarkably up-regulated in HCC and was associated with a poor survival. Knockdown of circNFIX repressed proliferation, invasion and glutaminolysis of HCC cells. Moreover, circNFIX directly sponged miR-3064-5p to release HMGA2 expression, and thus conferred the malignant development of HCC. In conclusion, circNFIX serves as a competing endogenous RNA to accelerate HCC progression via regulating miR-3064-5p/HMGA2 axis, suggesting a therapeutic strategy for HCC intervention.
Topics & Concepts
Gene knockdownCancer researchGlutaminolysisHepatocellular carcinomaCarcinogenesisHMGA2LuciferaseBiologyCell growthCancerMedicinemicroRNACancer cellInternal medicineCell cultureTransfectionGeneBiochemistryGeneticsCircular RNAs in diseasesCancer-related molecular mechanisms researchMicroRNA in disease regulation