Subcellular Detection of SARS-CoV-2 RNA in Human Tissue Reveals Distinct Localization in Alveolar Type 2 Pneumocytes and Alveolar Macrophages
Kofi Acheampong, Dylan L. Schaff, Benjamin Emert, Jonathan I. Lake, Sam Reffsin, Emily Shea, Courtney E. Comar, Leslie A. Litzky, Nigar Khurram, Rebecca L. Linn, Michael D. Feldman, Susan R. Weiss, Kathleen T. Montone, Sara Cherry, Sydney M. Shaffer
Abstract
RNA detection assay for RNA generated by the SARS-CoV-2 virus. We found viral RNA in lung, lymph node, and placenta samples from pathology specimens from COVID patients. Using high-magnification microscopy, we can visualize the subcellular distribution of these RNA in single cells.
Topics & Concepts
RNAAlveolar cellsBiologyIn situ hybridizationSubcellular localizationCell typeCoronavirusCellGene expressionVirologyGeneMolecular biologyLungCell biologyPathologyCytoplasmCoronavirus disease 2019 (COVID-19)GeneticsMedicineDiseaseInfectious disease (medical specialty)Internal medicineRespiratory viral infections researchSARS-CoV-2 detection and testingCOVID-19 Impact on Reproduction