Hypoxia-induced ribosomal RNA modifications in the peptidyl-transferase center contribute to anaerobic growth of bacteria
Kensuke Ishiguro, Karin Midorikawa, Naoki Shigi, Satoshi Kimura, Aivar Liiv, Takeshi Yokoyama, Takuhiro Ito, Mikako Shirouzu, Jaanus Rèmme, Kenjyo Miyauchi, Tsutomu Suzuki
Abstract
C2501) impaired anaerobic growth. Biochemical studies showed that these rRNA modifications stimulate protein synthesis. The cryoelectron microscopy (cryo-EM) structure of the ribosome indicated that these hypoxia-induced modifications stabilize the P-site and the PTC. These findings demonstrate that ribosomes are activated by hypoxia-induced modifications to enhance translational capability and thereby survival, under anaerobic conditions.
Topics & Concepts
BiologyRibosomeRibosomal RNA50SMethylationRibosomal proteinEscherichia coli5.8S ribosomal RNABiochemistryRNAAnaerobic exerciseAnaerobic bacteriaBacteriaTransfer RNAEukaryotic RibosomePeptidyl transferase30S23S ribosomal RNAMethyltransferaseCell biologyProtein biosynthesisProtein subunitMitochondrial ribosomeRiboseStrain (injury)5S ribosomal RNATranslation (biology)Ribosomal binding siteRNA methylationNon-coding RNAGeneticsRNA modifications and cancerBacterial Genetics and BiotechnologyRNA and protein synthesis mechanisms