Mitochondrial dysfunction and endoplasmic reticulum stress in calf hepatocytes are associated with fatty acid-induced ORAI calcium release-activated calcium modulator 1 signaling
Bingbing Zhang, Ming Li, Wei Yang, Juan J. Loor, Yusheng Liang, Shuang Wang, Yingying Zhao, Han Guo, Xinru Ma, Liyun Yu, Chuang Xu
Abstract
ATPase inhibitor thapsigargin or the calcium ionophore ionomycin. We detected an increase in VDAC1, CLPP, and CypD protein abundance, all of which indicated opening of the mPTP. Overall, data from these in vitro studies suggest that ORAI1 mediates ER stress induced by high concentrations of fatty acids, in part through alleviating mitochondrial dysfunction caused by oxidative stress.
Topics & Concepts
Unfolded protein responseEndoplasmic reticulumOxidative stressChemistryORAI1Reactive oxygen speciesThapsigarginLipotoxicityBiochemistryFatty acidCalciumCalcium in biologyCell biologySTIM1BiologyEndocrinologyIntracellularInsulinOrganic chemistryInsulin resistanceLiver Disease Diagnosis and TreatmentEndoplasmic Reticulum Stress and DiseaseAlcohol Consumption and Health Effects