Hidden hydrophobicity impacts polymer immunogenicity
Zhefan Yuan, Patrick McMullen, Sijin Luozhong, Pranab Sarker, Chenjue Tang, Tao Wei, Shaoyi Jiang
Abstract
immunogenicity for a polymer also applies to corresponding polymer-protein conjugates. Atomistic molecular dynamics (MD) simulation results show a similar trend. Based on polyzwitterion modification and with this HIC technique, we are able to produce extremely low-immunogenic protein conjugates as their hydrophilicity is pushed to the limit and their hydrophobicity is eliminated, breaking the current barriers of eliminating anti-drug and anti-polymer antibodies.
Topics & Concepts
ImmunogenicityPolymerPEG ratioEthylene glycolConjugated systemChemistryThermoresponsive polymers in chromatographyPEGylationPolymer architectureHydrophobic effectAntibodyChromatographyOrganic chemistryHydrophilic interaction chromatographyPolyethylene glycolHigh-performance liquid chromatographyBiologyFinanceImmunologyEconomicsProtein purification and stabilityMonoclonal and Polyclonal Antibodies ResearchMicrofluidic and Capillary Electrophoresis Applications