Identification of a CE-SDS shoulder peak as disulfide-linked fragments from common C<sub>H</sub>2 cleavages in IgGs and IgG-like bispecific antibodies
Mingyan Cao, Yang Jiao, Conner Parthemore, Samuel Korman, Jiao Ma, Alan K. Hunter, Greg W. Kilby, Xiaoyu Chen
Abstract
2 cleavages in antibodies, these findings will have broad applicability and could help manufacturers of therapeutic antibodies in process improvement, product characterization, investigations, formulation stability, and stability comparability studies.
Topics & Concepts
ChemistryAntibodyDisulfide bondCleavage (geology)Sodium dodecyl sulfateMolecular massChromatographyGel electrophoresisCapillary electrophoresisBiochemistryEnzymeBiologyGeotechnical engineeringImmunologyEngineeringFracture (geology)Protein purification and stabilityMonoclonal and Polyclonal Antibodies ResearchMicrofluidic and Capillary Electrophoresis Applications