Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay
Meng Liu, Rui Xu, Wenjing Liu, Jian‐Ge Qiu, Yan Wang, Fei Ma, Chun‐yang Zhang
Abstract
for caspase-9, and it can be used for the simultaneous screening of caspase inhibitors and the measurement of endogenous caspase activity in various cell lines at the single-cell level. Moreover, this nanosensor can be extended to detect various proteases by simply changing the peptide sequences of the detection probes.
Topics & Concepts
ExonucleaseDNAExonuclease IIIChemistryCaspaseMolecular biologyMoleculeCombinatorial chemistryBiophysicsCell biologyApoptosisBiochemistryBiologyDNA polymeraseGeneProgrammed cell deathOrganic chemistryEscherichia coliAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryBacteriophages and microbial interactions