IGF2 improves the developmental competency and meiotic structure of oocytes from aged mice
Tahir Muhammad, Yanling Wan, Qian‐Qian Sha, Jianfeng Wang, Tao Huang, Yongzhi Cao, Mengjing Li, Xiaochen Yu, Yingying Yin, Wai‐Yee Chan, Zi‐Jiang Chen, You Li, Gang Lü, Hongbin Liu
Abstract
< 0.05). Additionally, IGF2 supplementation of the culture medium reduced reactive oxygen species production and the incidence of spindle/chromosome defects. IGF2 increases mitochondrial functional activity in oocytes from aged mice: we detected increased ATP levels, elevated fluorescence intensity of mitochondria, higher mitochondrial membrane potentials, and increased overall protein synthesis, as well as increased autophagy activity and decreased apoptosis. Collectively, our findings demonstrate that IGF2 supplementation in culture media improves oocyte developmental competence and reduces meiotic structure defects in oocytes from aged mice.