Recent advances of single-cell RNA sequencing technology in mesenchymal stem cell research
Zheng Guan, Zhongyu Xie, Peng Wang, Yanfeng Wu, Huiyong Shen
Abstract
Mesenchymal stem cells (MSCs) are multipotent stromal cells with great potential for clinical applications. However, little is known about their cell heterogeneity at a single-cell resolution, which severely impedes the development of MSC therapy. In this review, we focus on advances in the identification of novel surface markers and functional subpopulations of MSCs made by single-cell RNA sequencing and discuss their participation in the pathophysiology of stem cells and related diseases. The challenges and future directions of single-cell RNA sequencing in MSCs are also addressed in this review.
Topics & Concepts
Mesenchymal stem cellStem cellCellRNABiologyComputational biologySingle-cell analysisBioinformaticsMedicineCell biologyGeneticsGeneMesenchymal stem cell researchCancer Cells and MetastasisSingle-cell and spatial transcriptomics