Homogeneous Dual-Parametric-Coupled Assay for Simultaneous Nucleotide Exchange and KRAS/RAF-RBD Interaction Monitoring
Kari Kopra, Emmiliisa Vuorinen, Maria Abreu Blanco, Qi Wang, Ville Eskonen, William Gillette, Arto T. Pulliainen, Matthew Holderfield, Harri Härmä
Abstract
molecule. The study indicates that the QTR-FRET detection technique presented here can be readily applied to dual-parametric assays for various targets.
Topics & Concepts
Förster resonance energy transferGuanine nucleotide exchange factorChemistryGTP'Heterotrimeric G proteinHigh-throughput screeningNucleotideGTPaseG proteinGuanineSmall moleculeSmall GTPaseQuenching (fluorescence)BiochemistryBiophysicsSignal transductionFluorescenceBiologyGenePhysicsQuantum mechanicsEnzymeReceptor Mechanisms and SignalingMonoclonal and Polyclonal Antibodies ResearchProtein Kinase Regulation and GTPase Signaling