Recent Advances in Improving Gene-Editing Specificity through CRISPR–Cas9 Nuclease Engineering
Xiaoqiang Huang, Dongshan Yang, Jifeng Zhang, Jie Xu, Y. Eugene Chen
Abstract
CRISPR-Cas9 is the state-of-the-art programmable genome-editing tool widely used in many areas. For safe therapeutic applications in clinical medicine, its off-target effect must be dramatically minimized. In recent years, extensive studies have been conducted to improve the gene-editing specificity of the most popular CRISPR-Cas9 nucleases using different strategies. In this review, we summarize and discuss these strategies and achievements, with a major focus on improving the gene-editing specificity through Cas9 protein engineering.
Topics & Concepts
CRISPRGenome editingCas9Computational biologyNucleaseComputer scienceGenetic enhancementGeneBiologyGeneticsCRISPR and Genetic EngineeringInnovation and Socioeconomic DevelopmentGenetics, Aging, and Longevity in Model Organisms