Active single cell encapsulation using SAW overcoming the limitations of Poisson distribution
Andreas Link, John S. McGrath, Mustafa Zaimagaoglu, Thomas Franke
Abstract
We demonstrate the use of an acoustic device to actively encapsulate single red blood cells into individual droplets in a T-junction. We compare the active encapsulation with the passive encapsulation depending on the number of loaded cells as well as the created droplet volumes. This method overcomes the Poisson limitation statistical loading of cells for the passive encapsulation. In our experiments we reach a single cell encapsulation efficiency of 97.9 ± 2.1% at droplet formation rates exceeding 15 Hz.
Topics & Concepts
Encapsulation (networking)Poisson distributionCell encapsulationMaterials scienceBiological systemNanotechnologyComputer scienceSelf-healing hydrogelsMathematicsComputer networkPolymer chemistryStatisticsBiologyInnovative Microfluidic and Catalytic Techniques InnovationMicrofluidic and Bio-sensing TechnologiesMicrofluidic and Capillary Electrophoresis Applications