The Spectrum of Spontaneous Rifampin Resistance Mutations in the Bacillus subtilis <i>rpoB</i> Gene Depends on the Growth Environment

Abstract

The rpoB gene encodes the beta subunit of RNA polymerase, and mutations in rpoB are key determinants of resistance to the clinically important antibiotic rifampin. We show here that the spectrum of mutations in Bacillus subtilis rpoB depends on the medium in which the cells are cultivated. The results show that the growth environment not only plays a role in natural selection and fitness but also influences the probability of mutation at particular bases within the target gene.

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