A Two-Layered Targeting Mechanism Underlies Nuclear RNA Sorting by the Human Exosome
Guifen Wu, Manfred Schmid, Leonor Rib, Patrik Polák, Nicola Meola, Albin Sandelin, Torben Heick Jensen
Abstract
3' ends defined by canonical pA site use. Irrespective of this clear division, NEXT and PAXT act redundantly in two ways: (1) regional redundancy, where the majority of exosome-targeted transcription units produce NEXT- and PAXT-sensitive RNA isoforms, and (2) isoform redundancy, where the PAXT connection ensures fail-safe decay of post-transcriptionally polyadenylated NEXT targets. In conjunction, this provides a two-layered targeting mechanism for efficient nuclear sorting of the human transcriptome.
Topics & Concepts
ExosomeMechanism (biology)SortingExosome complexRNACell biologyMicrovesiclesComputational biologyChemistryBiologymicroRNANon-coding RNAComputer scienceGeneticsPhysicsGeneQuantum mechanicsProgramming languageRNA Research and SplicingRNA modifications and cancerCancer-related molecular mechanisms research