Potential-Resolved Electrochemiluminescence Multiplex Immunoassay for Florfenicol and Chloramphenicol in a Single Sample
Yue Kong, Xiaolin Fan, Xun Yao, Kang Wu, Anping Deng, Xinjian Feng, Jianguo Li
Abstract
The simultaneous detection of multiple antibiotic residues in food is of great significance for food safety. In this work, a novel dual-potential electrochemiluminescence (ECL) immunoassay was designed for the simultaneous detection of chloramphenicol and fluorfenicol residues in food. Ru@MOF was used as an anodic probe, and SnS 2 QDs-PEI-Au-MoS 2 was used as a cathodic probe. Notably, the coreactant for both luminophores was K 2 S 2 O 8, avoiding interactions caused by different kinds of coreactants. Au nanoparticles functionalized with a nitrogen- and sulfur-doped graphene oxide-modified glassy carbon electrode to improve the electron transfer efficiency and provide a larger surface area for immobilization of antigen. The linear range for the detection of florfenicol was determined to be 0.1–1000 ng mL –1 with a detection limit of 0.03 ng mL –1, and the linear range for the detection of chloramphenicol was 0.01–1000 ng mL –1 with a detection limit of 3.2 pg mL –1 by recording the ECL responses at two different excitation potentials. The proposed immunoassay achieved a more stable recovery in the detection of actual samples and provided a new analytical method for the simultaneous detection of florfenicol and chloramphenicol residues with high sensitivity and specificity.