Redox state and altered pyruvate metabolism contribute to a dose-dependent metformin-induced lactate production of human myotubes
Jennifer Maurer, Xinjie Zhao, Martin Irmler, Anders Gudiksen, Nanna S. Pilmark, Qi Li, Thomas Goj, Johannes Beckers, Martin Hrabě de Angelis, Andreas L. Birkenfeld, Andreas Peter, Rainer Lehmann, Henriette Pilegaard, Kristian Karstoft, Guowang Xu, Cora Weigert
Abstract
Metformin shifts the equilibrium of lactate dehydrogenase (LDH) reaction by low dose-induced phosphorylation of pyruvate dehydrogenase (PDH) resulting in inhibition of pyruvate oxidation and high dose-induced increase in NADH, which explains the dose-dependent lactate production of differentiated human skeletal muscle cells.
Topics & Concepts
MetforminRedoxMetabolismMyogenesisChemistryCell biologyBiochemistryEndocrinologyBiologyIn vitroDiabetes mellitusOrganic chemistryMetabolism, Diabetes, and CancerPancreatic function and diabetesBiochemical Acid Research Studies