Field Performance and Public Health Response Using the BinaxNOWTM Rapid Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Antigen Detection Assay During Community-Based Testing
Genay Pilarowski, Carina Marquez, Luis Rubio, James Peng, Jackie Martinez, Douglas Black, Gabriel Chamie, Diane Jones, Jon Jacobo, Valerie Tulier-Laiwa, Susana Rojas, Susy Rojas, Chesa Cox, Robert M. Nakamura, Maya Petersen, Joseph L. DeRisi, Diane V. Havlir
Abstract
Among 3302 persons tested for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by BinaxNOWTM and reverse transcription polymerase chain reaction (RT-PCR) in a community setting, rapid assay sensitivity was 100%/98.5%/89% using RT-PCR cycle thresholds of 30, 35, and no threshold. The specificity was 99.9%. Performance was high across ages and those with and without symptoms. Rapid resulting permitted immediate public health action.
Topics & Concepts
MedicineSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)VirologyCoronavirusRespiratory systemCoronavirus disease 2019 (COVID-19)Reverse transcription polymerase chain reactionAntigenSevere acute respiratory syndrome coronavirus2019-20 coronavirus outbreakPolymerase chain reactionImmunologyInternal medicineGeneInfectious disease (medical specialty)DiseaseBiologyMessenger RNABiochemistryOutbreakSARS-CoV-2 detection and testingBiosensors and Analytical DetectionRespiratory viral infections research