Radiofrequency Driving Antitumor Effect of Graphene Oxide-Based Nanocomposites: A Hill Model Analysis
Melissa Silva Monteiro, Marina M. Simões, L.M. García, Paulo Roberto Santos, C. Viveiros, Ronei Delfino da Fonseca, Mary-Ann Xavier, Gabriel WS de Mendonça, Suélia de Siqueira Rodrigues Fleury Rosa, S. Silva, Leonardo G. Paterno, P.C. Morais, Sônia Nair Báo
Abstract
Aim: This report proposes using the Hill model to assess the benchmark dose, the 50% lethal dose, the cooperativity and the dissociation constant while analyzing cell viability data using nanomaterials to evaluate the antitumor potential while combined with radiofrequency therapy. Materials & methods: A nanocomposite was synthesized (graphene oxide–polyethyleneimine–gold) and the viability was evaluated using two tumor cell lines, namely LLC-WRC-256 and B16-F10. Results: Our findings demonstrated that while the nanocomposite is biocompatible against the LLC-WRC-256 and B16-F10 cancer cell lines in the absence of radiofrequency, the application of radiofrequency enhances the cell toxicity by orders of magnitude. Conclusion: This result points to prospective studies with the tested cell lines using tumor animal models.