Development of a Quorum Sensing-Mediated Bacterial Autolytic System in <i>Escherichia coli</i> for Automatic Release of Intracellular Products
Xiaofei Song, Yifan Zhao, Yixuan Ren, Ruo-Yu Liu, Mengting Zhang, Zhikai Zhang, Qiu Meng, Tingheng Zhu, Jianhua Yin, Zhiliang Yu
Abstract
Escherichia coli, one of the most efficient expression hosts for recombinant proteins, is widely used in chemical, medical, food, and other industries. De novo engineering of gene regulation circuits and cell density-controlled E. coli cell lysis are promising directions for the release of intracellular bioproducts. Here, we developed an E. coli autolytic system, named the quorum sensing-mediated bacterial autolytic (QS-BA) system, by incorporating an acyl-homoserine lactone (AHL)-based YasI/YasR-type quorum sensing circuit from Pseudoalteromonas into E. coli cells. The results showed that the E. coli QS-BA system can release the intracellular bioproducts into the cell culture medium in terms of E. coli cell density, which offers an environmentally-friendly, economical, efficient, and flexible E. coli lysis platform for production of recombinant proteins. The QS-BA system has the potential to serve as an integrated system for the large-scale production of target products in E. coli for medical and industrial applications.