Standardization of Synthetic Biology Tools and Assembly Methods for <i>Saccharomyces cerevisiae</i> and Emerging Yeast Species
Koray Malcı, Emma C. Watts, Tania Michelle Roberts, Jamie Auxillos, Behnaz Nowrouzi, Heloísa Oss Boll, Cibele Zolnier Sousa do Nascimento, Andreas Andreou, Péter Végh, Sophie Donovan, Rennos Fragkoudis, Sven Panke, Edward W. Wallace, Alistair Elfick, Leonardo Rios‐Solis
Abstract
are also reviewed. Without a doubt, the characterized DNA parts combined with the standardized assembly strategies highlighted in these toolkits have greatly contributed to the rapid development of many metabolic engineering and diagnostics applications among others. Despite the growing capacity in deploying synthetic biology for common yeast genome engineering works, the yeast community has a long journey to go to exploit it in more sophisticated and delicate applications like bioautomation.