Optimization of microwave-assisted hydrothermal extraction of homogalacturonan-rich, low methoxyl pectin from discarded garlic peels: Comparative macromolecular and functional analysis with conventional extraction
Diego J. González-Serrano, Imed E Benmebarek, Milad Hadidi, Julia Bechtner, Mario M. Martínez, Beatriz Cabañas, Andrés Moreno
Abstract
This study explored the extraction of pectin from garlic peels and its potential application as a food hydrocolloid due to its liquid-holding, emulsifying, thickening, and gelling properties. A Box-Behnken optimization of microwave-assisted hydrothermal extraction (MAHE) was performed to examine the effects of pH, temperature, and irradiation time on pectin yield (PY), galacturonic acid concentration (GalAC), degree of methylation (DM), and degree of acetylation (DA). Low methoxyl (36.1% DM) and low-acetyl (0.9% DA) pectin (MAHEP) were obtained at optimal MAHE conditions, achieving a high GalAC (74.9%) and a notable PY (14.5%). Following MAHE optimization, a two-hour conventional heat extraction (CE) was conducted under the same temperature, pH, and ratio. Pectin obtained through CE (CEP) demonstrated a lower PY (12.1%) but maintained a high purity level (67.1%) and exhibited higher DM (46.6%) and DA values (1.1%). Both MAHEP and CEP were rich in homogalacturonan (HG) domains (83.6–92.2% HG ratio) but differed in DM, thermal degradation behavior, and functionality. CEP showed excellent water retention (9.0 g/g) and thickening properties (2.5 Pa·s at 4% w/w and 1 s -1 ), whereas MAHEP excelled in emulsifying stability (85–99% under all conditions tested) as well as gelling performance (G´ > G’’ for all frequencies tested at 4% w/w). This study highlights the potential of garlic peels as a source of two distinct pectic polysaccharides with high purity and functional versatility. It also provides insights into how the extraction system influences pectin structure and functional behavior. • Garlic peels rendered low methoxyl pectin very rich in homogalacturonan domain. • Microwave-assisted hydrothermal extraction (MAHE) was optimized for four responses. • Garlic-derived pectin with high purity and 14.5% yield was optimally obtained. • Conventionally extracted pectin showed good water holding and thickening behavior. • MAHE pectin showed remarkable emulsifying stability and gelling properties.