A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases
Lijuan Wang, Le Liang, Bingjie Liu, Bing‐Hua Jiang, Chun‐yang Zhang
Abstract
for hAAG, and it can even quantify repair glycosylases at the single-cell level. Moreover, it can be applied for the measurement of kinetic parameters, the screening of potential inhibitors, and the detection of repair glycosylases in human serum, providing a new paradigm for repair enzyme-related biomedical research, drug discovery, and clinical diagnosis.
Topics & Concepts
CascadeDNA glycosylaseTranscription (linguistics)ChemistryComputational biologyCell biologyBiologyComputer scienceGeneticsGeneDNA repairChromatographyPhilosophyLinguisticsAdvanced biosensing and bioanalysis techniquesCytomegalovirus and herpesvirus researchCRISPR and Genetic Engineering