Discovery of branching meroterpenoid biosynthetic pathways in <i>Aspergillus insuetus</i>: involvement of two terpene cyclases with distinct cyclization modes

Abstract

strain also harbors an additional gene cluster encoding another cyclase of 1. The second terpene cyclase-InsB2-also synthesizes a new cyclized product of 1, thereby leading to diverging of the biosynthetic pathway in the fungus. Finally, we characterized the tailoring enzymes encoded by the two clusters, collectively obtained 17 new meroterpenoids, and successfully proposed biosynthetic routes leading to apparent end products of both pathways.

Topics & Concepts