Arginine-induced metabolomic perturbation in <i>Streptococcus mutans</i>
Yudong Liu, Shanshan Liu, Qinghui Zhi, Peilin Zhuang, Rongxiu Zhang, Zhenzhen Zhang, Kai Zhang, Yu Sun
Abstract
BACKGROUND: growth remains unclear. AIM: under biofilm conditions. METHODS: We identified 5,933 and 7,413 ions in positive (ESI+) and negative (ESI-) electrospray ion modes, respectively, with a total of 11.05% and 11.58% differential ions subsequently detected in two respective modes. Further analyses of these metabolites led to identification of 8 and 22 metabolic pathways that were affected by arginine treatment in ESI+ and ESI- modes. RESULTS: biofilms with arginine resulted in reductions in biofilm biomass. Significant reductions in EPS production were observed following twice daily arginine treatments. Identified metabolites that were significantly differentially abundant following arginine treatment were associated with glycolysis metabolism, amino sugar and nucleotide sugar metabolism, and peptidoglycan synthesis. CONCLUSIONS: biofilm growth and acid production by inhibiting glycolysis, amino sugar and nucleotide sugar metabolism, and peptidoglycan synthesis.