Prediction model for malignant pulmonary nodules based on cfMeDIP‐seq and machine learning
Jian Qi, Bo Hong, Rui Tao, Ruifang Sun, Huanhu Zhang, Xiaopeng Zhang, Jie Ji, Shujie Wang, Yanzhe Liu, Qingmei Deng, Hongzhi Wang, Dahai Zhao, Jinfu Nie
Abstract
Cell-free methylated DNA immunoprecipitation and high-throughput sequencing (cfMeDIP-seq) is a new bisulfite-free technique, which can detect the whole-genome methylation of blood cell-free DNA (cfDNA). Using this technique, we identified differentially methylated regions (DMR) of cfDNA between lung tumors and normal controls. Based on the top 300 DMR, we built a random forest prediction model, which was able to distinguish malignant lung tumors from normal controls with high sensitivity and specificity of 91.0% and 93.3% (AUROC curve of 0.963). In summary, we reported a non-invasive prediction model that had good ability to distinguish malignant pulmonary nodules.