Antimicrobial, and Antibiofilm Activities of Silver Doped Hydroxyapatite: A Novel Bioceramic Material for Dental Filling
Sherif Elbasuney, Gharieb S. El‐Sayyad, Sara M. Radwan, Miguel A. Correa‐Duarte
Abstract
Abstract Even though hydroxyapatite (HA) is the most common biocompatible material; it has limited antibacterial resistance. HA experiences a tailor-made structure depending on the desired applications. In this regard, silver (Ag) is of particular interest to inhibit wide spectrum of pathogenic bacteria and other microorganisms. Silver doped hydroxyapatite (Ag-HA) was developed via wet co-precipitation with subsequent hydrothermal processing to hinder the growth and multiplication of pathogenic microbes. Ag-HA demonstrated mono-dispersed nano-rods of 70 nm length and 7 nm diameters. Even though silver dopant induced stresses within the crystal lattice; Ag-HA maintained the crystallographic structure of HA with no change. Ag-HA nanocomposite demonstrated Ca/P value of 1.238 compared with 1.402 for virgin HA via XPS spectroscopy. The reduction of Ca/P value was correlated to the partial replacement of Ca +2 with Ag +1 ; Silver content was reported to be of 1 atomic %. Elemental mapping using EDAX confirmed uniform dispersion of silver ion within HA lattice. Antimicrobial results indicated that, Ag-HA nanocomposite demonstrated the most potent zone of inhibition (ZOI) against Staphylococcus aureus , and Candida albicans . Antibiofilm results indicated that Ag-HA nanocomposite at 10.0 µg/mL, experienced the highest percentage for S. aureus and C. albicans of 96.09%, and 77.77%, respectively. Ag-HA nanocomposite demonstrated an excellent disinfectant agent once it had excited by UV light. In growth curve assay, the OD 600 value of Ag- HA were lower, showing the repression impact on the growth of S. aureus . It was observed that the quantity of cellular protein discharged from S. aureus is directly proportional to the concentration of Ag-HA, which proves the antibacterial characteristics of the Ag-HA nanocomposite, and explains the creation of holes in the bacterial membrane producing the oozing out of the proteins from the S. aureus cytoplasm. Ag-HA nanocomposite achieved the complete lysis of the bacterial cell and cell malformation, decreasing the total viable number, so the outstanding antimicrobial results encouraged the tooth filling biomedical applications. Graphical Abstract